Coding

Part:BBa_K1422507:Design

Designed by: Casey Martin   Group: iGEM14_Purdue   (2014-10-17)


Zea mays TOM1 (corn phytosiderophore transporter)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 98
    Illegal EcoRI site found at 112
    Illegal XbaI site found at 754
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 98
    Illegal EcoRI site found at 112
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 98
    Illegal EcoRI site found at 112
    Illegal BglII site found at 470
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 98
    Illegal EcoRI site found at 112
    Illegal XbaI site found at 754
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 98
    Illegal EcoRI site found at 112
    Illegal XbaI site found at 754
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 961
    Illegal BsaI.rc site found at 1334


Design Notes

Codon optimized for Bacillus subtilis. The 60th, 283rd, 293rd, and 295th codons were altered from CTA to CTT.


Source

The original, unaltered gene sequence came from NCBI. NCBI. (10 December, 2008). Zea mays clone 222622 protein transporter mRNA, complete cds. http://www.ncbi.nlm.nih.gov/nuccore/195621011

References